Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody

About this product

Boster Bio Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody (Catalog # M01063) . Tested in IHC applications. This antibody reacts with Human.

Specifications

Catalog number

M01063

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Synonyms

Filaggrin; FLG

Gene Name

FLG

UniProt

P20930

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

Clone: Clone: FLG/1562

Tissue Specificity

Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417) . In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417) . .

Applications

IHC

Field of Research

Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Signal Transduction, Tags & Cell Markers

Purification

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.

Concentration

Purified antibody with BSA and azide at 200ug/ml

Form

Liquid

Function

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Molecular Weight

435170 MW

Shipping Conditions

Available

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Fragment

IgG1, kappa

Other Gene Names

Filaggrin

Subcellular Location

Cytoplasmic