Anti-HSP60 HSPD1 Monoclonal Antibody

About this product

Boster Bio Anti-HSP60 HSPD1 Monoclonal Antibody catalog # M01280-2. Tested in ELISA, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01280-2

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3) . Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4) . These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5) . Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9) .

Synonyms

CPN60, GROEL, HLD4, HSP 60, HSP65, HSPD1, HuCHA60, SPG 13

Gene Name

HSP60

UniProt

P10809

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken, Drosophila, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus, Plant, Silkworm

Cross Reactivity

Detects ~60kDa.

Immunogen

Recombinant human HSP60

Clonality

Monoclonal

Clone

Clone: LK1

Applications

WB, IHC, IP, ELISA, Flow Cytometry

Field of Research

Chaperones, Heat Shock Proteins, Mitochondria, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200) ; optimal dilutions for assays should be determined by the user.

Form

Liquid

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

IgG1

Subcellular Location

Mitochondrion. Mitochondrion Matrix

Protein Name

HSP60