Anti-FKBP52 Monoclonal Antibody

About this product

Boster Bio Anti-FKBP52 Monoclonal Antibody catalog # M02165-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02165-1

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1) . One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2) . FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3) . The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4) . Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3) . FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5) .

Synonyms

FK506 binding protein 4, FKBP4, FKBP59, HBI, HSP56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein

Gene Name

FKBP52

UniProt

Q02790

Host

Mouse

Reactivity

Human, Mouse, Rat, Dog, Hamster

Cross Reactivity

Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Immunogen

Synthetic peptide corresponding to the residues of human FKBP52

Clonality

Monoclonal

Clone

Clone: Hi52C

Applications

WB, IHC, ICC, IF, IP

Field of Research

Chaperones, Cytoskeleton, Cytoskeleton/ECM, Epigenetics and Nuclear Signaling, Heat Shock Proteins, Microtubules, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Protein Trafficking, Signal Transduction, Signaling Pathway, Tubulin

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg) ; optimal dilutions for assays should be determined by the user.

Form

Liquid

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

IgG2a

Subcellular Location

Cytoplasm. Nucleus

Protein Name

FKBP52