Anti-GRP94 Monoclonal Antibody

About this product

Boster Bio Anti-GRP94 Monoclonal Antibody catalog # M02393-2. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02393-2

Size / count

200 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1) . Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2) . Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3) . Both HSP90 and Grp94 are calcium binding proteins (4) . Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5) . Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6) . Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7) .

Synonyms

HSP90B1, GP96, TRA1, ECGP, 94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL_HUMAN, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 94, GRP-94, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, Heat shock protein, 90 kDa, beta, 1, HSP90B1, Stress inducible tumor rejection antigen GP96, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96)

Gene Name

GRP94

UniProt

P14625

Host

Rat

Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea pig, Hamster, Horse, Monkey, Pig, Rabbit, Sheep, Xenopus

Cross Reactivity

Detects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.

Immunogen

Purified Grp94 isolated from chicken oviducts

Clonality

Monoclonal

Clone

Clone: 9G10

Applications

WB, ICC, IF, IP, Flow Cytometry

Field of Research

Tags & Cell Markers

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Form

Liquid

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

IgG2a

Subcellular Location

Endoplasmic Reticulum| Endoplasmic Reticulum Lumen. Melanosome

Protein Name

GRP94