Anti-Ubiquitin Monoclonal Antibody

About this product

Boster Bio Anti-Ubiquitin Monoclonal Antibody catalog # M02848-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, C. elegans, Chicken, Cow, Drosophila, Equine, Human, Monkey, Mouse, Pig, Rat, Horse.

Specifications

Catalog number

M02848-4

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1; Ubiquitin;60S ribosomal protein L40; Large ribosomal subunit protein eL40 ; UBA52; UBCEP2

Gene Name

UBA52

UniProt

P62987

Host

Mouse

Reactivity

Bovine, C. elegans, Chicken, Cow, Drosophila, Equine, Human, Monkey, Mouse, Pig, Rat, Horse

Immunogen

Purified bovine erythrocyte ubiquitin coupled to KLH with glutaraldehyde

Clonality

Monoclonal

Applications

IF, IHC, ICC, WB

Field of Research

Cell Biology, DNA/RNA, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ribosome, Translation

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1,000-1:2,000. ICC/IF and IHC: 1:2,000

Function

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored) . When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains) . Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Molecular Weight

14728 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Other Gene Names

Ubiquitin-60S ribosomal protein L40

Subcellular Location

Ubiquitin: Cytoplasm . Nucleus .