Anti-CaMKII (Alpha-Specific) Monoclonal Antibody

About this product

Boster Bio Anti-CaMKII (Alpha-Specific) Monoclonal Antibody catalog # M03241-1. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M03241-1

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2) . CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes) . CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7) . The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8) . This kinase activation results in autophosphorylation at threonine 286 (8) . The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9) . Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12) .

Synonyms

CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA

Gene Name

CaMKII

UniProt

P11798

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine

Cross Reactivity

Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Immunogen

Partially purified rat CaMKII

Clonality

Monoclonal

Clone

Clone: 6G9

Applications

WB, IHC, ICC, IF, IP, ELISA, RIA

Field of Research

Calcium Signaling, Calmodulin Pathway, Cardiovascular, Hypertrophy, Neuroscience, Neurotransmission, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:10000), IHC (1:2000), ICC/IF (1:50) ; optimal dilutions for assays should be determined by the user.

Form

Liquid

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

IgG1

Subcellular Location

Cytoplasm. Mitochondrion. Nucleus. Cell Junction. Synapse. Presynaptic Cell Membrane

Protein Name

CaMKII