Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody

About this product

Boster Bio Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody catalog # M04133. Tested in IF, ICC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M04133

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

MARCKS-related protein; Brain protein F52; MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate; Mac-MARCKS; MacMARCKS; Marcksl1; Mlp, Mrp

Gene Name

Marcksl1

UniProt

P28667

Host

Rabbit

Reactivity

Human

Immunogen

Full length recombinant human MARCKS purified from E. coli

Clonality

Polyclonal

Tissue Specificity

Expressed at high levels in brain cortex and hippocampus (at protein level), including dentate gyrus, anterior olfactory nucleus, primary olfactory cortex, entorhinal cortex, medial preoptic area and dorsomedial hypothalamic nucleus. Expressed in neuronal cells (at protein level) . Also detected in the retina. Up-regulated in a wide variety of cancers. .

Applications

WB, IF, ICC

Field of Research

Calcium Signaling, Calmodulin Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway

Sensitivity

<50pg/ml

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

Western blot: 1:10,000-1:20,000. IF/ICC and IHC: 1:500-1:1,000.

Function

Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems. .

Molecular Weight

20165 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Other Gene Names

MARCKS-related protein

Subcellular Location

Cytoplasm. Cell membrane ; Lipid-anchor . Associates with the membrane via the insertion of the N-terminal N-myristoyl chain and the partial insertion of the effector domain. Association of the effector domain with membranes may be regulated by Ca (2+) /calmodulin.