Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248

About this product

Boster Bio Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (Catalog # M04635-1) . Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.

Specifications

Catalog number

M04635-1

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Core histone macro-H2A.1; Histone macroH2A1; mH2A1; H2A.y; H2A/y; H2afy

Gene Name

H2afy

UniProt

Q9QZQ8

Host

Rabbit

Reactivity

Human, Vertebrates

Cross Reactivity

This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.

Immunogen

A peptide corresponding to the C-terminus of human Histone macroH2A1.

Clonality

Monoclonal

Clone

Clone: RM248

Tissue Specificity

Widely expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level) . In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, expressed in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.

Applications

WB, ICC, ELISA, Luminex

Field of Research

2339, Epigenetics and Nuclear Signaling, Histones

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

1 mg/mL

Form

Liquid

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (By similarity) . Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (By similarity) (PubMed:16107708) .

Molecular Weight

39735 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Core histone macro-H2A.1

Subcellular Location

Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin (PubMed:9634239) . In quiescent lymphocytes, associated with centromeric constitutive heterochromatin (PubMed:15564378) .