Anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Monoclonal Antibody

About this product

Boster Bio Anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Monoclonal Antibody (Catalog # M09702) . Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

M09702

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Synonyms

DNA topoisomerase I, mitochondrial; TOP1mt;5.99.1.2; TOP1MT

Gene Name

TOP1MT

UniProt

Q969P6

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human TOP1MT protein

Clonality

Monoclonal

Clone

Clone: Clone: TOP1MT/488

Tissue Specificity

Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver. .

Applications

Flow Cytometry, IF, WB, IHC

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.

Concentration

Purified antibody with BSA and azide at 200ug/ml

Form

Liquid

Function

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) . .

Molecular Weight

69872 MW

Shipping Conditions

Available

Storage Conditions

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Fragment

IgG2b, kappa

Other Gene Names

DNA topoisomerase I, mitochondrial

Subcellular Location

Cytoplasm (Mitochondria)