- Catalog number
- P00279-1
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. <br>
<br>
Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394. <br>
Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715. <br>
R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545. <br>
Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.
- Synonyms
- Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin; NF2; SCH
- Gene Name
- NF2
- UniProt
- P35240
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Immunogen
- Peptide sequence around phosphorylation site of serine 518 (R-L-S (p) -M-E) derived from Human Merlin.
- Clonality
- Polyclonal
- Tissue Specificity
- Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
- Applications
- WB, IF
- Field of Research
- Epigenetics and Nuclear Signaling, Intracellular Signaling, Membrane Proteins, Neuroscience, Neurotransmission, Oncoproteins/Suppressors, Signal Transduction, Transcription, Tumor Suppressors
- Purification
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Concentration
- 1 mg/ml
- Form
- Liquid
- Function
- Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. .
- Molecular Weight
- 69690 MW
- Shipping Conditions
- Available
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Other Gene Names
- Merlin
- Subcellular Location
- Isoform 1: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.
