Anti-Phospho-Troponin I, cardiac (Ser43) Antibody

About this product

Boster Bio Anti-Phospho-Troponin I, cardiac (Ser43) Antibody (Catalog # P01720-2) . Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

P01720-2

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995) . Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser-43 and Ser-45 of cardiac troponin I plays a substantial role in the PKC mediated depression. (Montgomery et al, 2002) .

Phospho Site

Phosphorylated, Ser43

Synonyms

Cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody

Gene Name

TNNI3

UniProt

P48787

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Mouse.

Clonality

Polyclonal

Applications

WB

Field of Research

Cancer, Cardiovascular, Metabolism

Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

WB: 1:1000

Form

Liquid

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Molecular Weight

24008 MW

Shipping Conditions

Available

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Fragment

IgG

Applications Notes

Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43. Immunolabeling is greatly decreased with λ-phosphatase treatment.

Other Gene Names

Troponin I, cardiac muscle

Prediction Reactivity

Bovine, Canine, Primate, Sheep, Xenopus, Zebrafish

Protein Name

Troponin I, cardiac muscle