- Catalog number
- P07297
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-B receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. Phosphorylation enhances GABA-B receptor effector coupling (Couve et al., 2004) . Phosphorylation of Ser-923 is thought to be important in the regulation of GABA-B receptor function.
- Phospho Site
- Phosphorylated, Ser923
- Synonyms
- DJ271M21.1.1 antibody, dJ271M21.1.2 antibody, FLJ92613 antibody, GABA-B receptor 1 antibody, GABA-B-R1 antibody, GABA-BR1 antibody, GABAB R1 antibody, GABAB subunit 1c antibody, GABABR1 antibody, GABBR1 3 antibody, GABBR1 antibody, GABR1_HUMAN antibody, Gamma aminobutyric acid (GABA) B receptor 1 antibody, Gamma-aminobutyric acid type B receptor subunit 1 antibody, Gb1 antibody, GPRC3A antibody, Seven transmembrane helix receptor antibody
- Gene Name
- GABBR1
- UniProt
- Q9Z0U4
- Host
- Rabbit
- Reactivity
- Mouse, Rat
- Immunogen
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser923 of the R1-subunit of rat GABAB receptor, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.
- Clonality
- Polyclonal
- Tissue Specificity
- Isoform 1A, isoform 1B and isoform 1C are expressed in testis, stomach, spinal cord and brain including cerebral cortical layers, pyramidal cell layers of the hippocampus, granular cell layers of the dentate gyrus, basal ganglia, cerebellum (predominantly in Purkinje cells followed by granular layer) . Isoform 1B is also expressed in kidney and liver. Isoform 1D is expressed in forebrain, cerebellum, eye, kidney, and urinary bladder.
- Applications
- WB
- Purification
- Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Dilution
- WB: 1:1000
- Form
- Liquid
- Function
- Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
- Molecular Weight
- 111534 MW
- Shipping Conditions
- Available
- Storage Conditions
- Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
- Fragment
- IgG
- Applications Notes
- Specific for endogenous levels of the ~102 kDa GABAB R1 phosphorylated at Ser923. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
- Other Gene Names
- Gamma-aminobutyric acid type B receptor subunit 1
- Subcellular Location
- Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes.
- Prediction Reactivity
- Bovine, Human, Primate
- Protein Name
- Gamma-aminobutyric acid type B receptor subunit 1
