DNA Damage (8-OHdG) ELISA Kit

About this product

Colorimetric detection of DNA Damage (8-OHdG) . 96 wells/kit.

Specifications

Catalog number

EK7114

Size / count

96 Well/Kit

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species) . Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS.

Synonyms

8-OH-dG; 8OHG; 80G; 8 hydroxyguanine; 8-OHdG; DNA Damage

Gene Name

DNA Damage (8-OHdG)

Reactivity

All species

Cross Reactivity

8-Hydroxy-2-deoxy Guanosine (8-OHdG) : 100%. 8-Hydroxy Guanosine (8-OHG) : 23%. 8-Hydroxy Guanine (8-oxoG) : 23%. Guanosine: <0.01%.

Applications

ELISA

Sample Type

Urine, Cell Lysates, Plasma, Sample matrices

Detection Range

0.94 - 60 ng/mL

Sensitivity

0.59 ng/mL

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Protein Name

DNA Damage (8-OHdG)