CD73 Recombinant Mouse Monoclonal Antibody [A6G2-R]

About this product

5′-nucleotidase (5′-NT), also known as ecto-5′-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain. NT5E can act as an immune inhibitory control molecule. Free adenosine generated by NT5E inhibits cellular immune responses and thereby promotes immune escape of tumor cells.

Specifications

Catalog number

HA601299

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

5' NT antibody 5' nucleotidase (CD73) antibody 5' nucleotidase precursor antibody 5' nucleotidase, ecto antibody 5' nucleotidase, ecto (CD73) antibody 5'-NT antibody 5'-nucleotidase antibody 5NTD_HUMAN antibody CD73 antibody CD73 antigen antibody E5NT antibody Ecto 5' nucleotidase antibody Ecto-5'-nucleotidase antibody eN antibody eNT antibody NT antibody NT5 antibody NT5E antibody NTE antibody Purine 5 Prime Nucleotidase antibody

Abbreviation

NT5E

Swiss Prot

P21589 Human, Q61503 Mouse, P21588 Rat

Cellular Locus

Cell membrane.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human CD73 27-549.

Isotype

IgG1

Clone

A6G2-R

Conjugation

Non-conjugated

Type

Recombinant Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

U-87 MG cell lysate, NCI-H441 xenograft tissue, U-87 MG xenograft tissue, human liver tissue, mouse liver tissue, rat liver tissue, U-87 MG.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 63 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes