Recombinant Mouse TNF RII Protein (hIgG1 Fc Tag)

Specifications

Catalog number

PDMM100048-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Abbreviation

TNF RII

Synonyms

Tumor necrosis factor receptor; CD120b; p75; p75TNFR; p80 TNF-alpha receptor; TBPII; TNFBR; TNFR1B; TNF-R2; TNF-R75; TNFR80; TNFR-II; TNF-RII; TNF-R-II; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tnfr-2; Tnfr2; Tnfrsf1b; p80; TNFRSF1b; TNFalpha-R2; TNF-alphaR2; Tnfr-1; TNFRII

UniProt

P25119

Accession Number

P25119

Expression System

Mammalian

Tag

C-hIgG1 Fc

Sequence

Val23-Gly258

Field of Research

Immunology; Signal Transduction; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.9 kDa

Observed Molecular Weight

55-65 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse