Phospho-RIP3 (S232) Recombinant Rabbit Monoclonal Antibody [JE44-62]

About this product

Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (By similarity). In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to flavivirus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate. Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL (By similarity). These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production (By similarity).

Specifications

Catalog number

HA721428

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Receptor interacting protein 3 antibody Receptor interacting serine threonine kinase 3 antibody Receptor interacting serine/threonine protein kinase 3 antibody Receptor-interacting protein 3 antibody Receptor-interacting serine/threonine-protein kinase 3 antibody RIP 3 antibody RIP like protein kinase 3 antibody RIP-3 antibody RIP-like protein kinase 3 antibody RIPK 3 antibody RIPK3 antibody RIPK3_HUMAN antibody

Abbreviation

Ripk3, Rip3

Swiss Prot

Q9QZL0 Mouse, Q9Z2P5 Rat

Cellular Locus

Cytoplasm, cytosol, Nucleus.

Host

Rabbit

Species Reactivity

Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser232 of Human RIP3.

Isotype

IgG

Clone

JE44-62

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

L-929 treated with 20μM Z-VAD for 3.5 hours add 100nM SM-164 and 20ng/ml TNF-α for 3 hours whole cell lysate, L-929 cells treated with 20μM Z-VAD for 2.75 hours, add 100nM SM-164 and 20ng/ml TNF-α for 2.25 hours.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes