AF/LE Purified Anti-Human CD11a Antibody[HI111]

Specifications

Catalog number

E-AB-F13160-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50) .

Abbreviation

CD11a

Gene ID

3683

UniProt

P20701

Host

Mouse

Reactivity

Human

Target

LFA; Lymphocyte antigen; CD11A; LFA-1; LFA1A; ITGAL; LFA-1A; Ly-15; Lymphocyte antigen 15; Integrin alpha-L; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain (LFA-1A) ; Leukocyte function-associated molecule 1 alpha chain; integrin &alpha-L-L; LFA1

Clonality

Monoclonal

Clone

HI111

Conjugation

None (AF/LE)

Applications

FCM

Field of Research

Signal Transduction; Immunology; Stem Cells

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype

Mouse IgG1, κ