QuicKey Pro Rat ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit

Specifications

Catalog number

E-OSEL-R0012-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ACE2

UniProt

Q5EGZ1

Cross Reactivity

This kit recognizes ACE2 in samples.No significant cross-reactivity or interference between ACE2 and analogues was observed.

Target

ACEH; ACEII

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ACE2 are added to the micro ELISA plate wells. Rat ACE2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ACE2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE2. The concentration of Rat ACE2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.39-25 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.23 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Detection Instrument

Microplate reader

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat ACE2 concentrations in serum, plasma and other biological fluids.