Phospho-CHEK1 (S280) antibody

Specifications

Catalog number

FNab09816

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Synonyms

Serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Cell cycle checkpoint kinase|Checkpoint kinase-1|CHEK1|CHK1

Gene ID

1111

UniProt

O14757

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Phospho-CHEK1-S280

Target

Phospho-CHEK1 (S280)

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB, IHC, IF

Purification

Immunogen affinity purified

Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

Purity

≥95% as determined by SDS-PAGE

Form

Liquid

Molecular Weight

60 kDa

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Tested Applications

ELISA, WB, IHC, IF