LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82]

Catalog number HA723034

Price for HA723034

Size / count 100 µL

About this product

LIM domain kinase 2 is an enzyme that in humans is encoded by the LIMK2 gene. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Specifications

Catalog number: HA723034
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: LIM domain kinase 2 antibody LIM kinase 2 antibody LIMK 2 antibody LIMK-2 antibody Limk2 antibody LIMK2_HUMAN antibody
Abbreviation: LIM domain kinase 2 LIMK-2 LIMK2
Swiss Prot: P53671 Human, O54785 Mouse, P53670 Rat
Cellular Locus: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Monkey
Immunogen: Recombinant protein within human LIMK2 aa 1-320.
Isotype: IgG
Clone: PSH08-82
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, IP
Positive Control: Ramos cell lysate, Jurkat cell lysate, HeLa cell lysate, SW480 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, Ranos, PC-12, human colon tissue, human colon cancer tissue, human pancreas tissue, mouse colon tissue, mouse pancreas tissue, rat colon tissue, rat pancreas tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:2,000 ;IF-Cell: 1:50 ;IHC-P: 1:1,000 ;IP: 1-2μg/sample
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 72 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82]?

The catalog number for LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82] is HA723034.

What sizes are available for LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82]?

LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82] is available in the following size(s): 100 µL.

Is LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82] in stock?

LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82] is currently made to order / not held in immediate stock. Contact us for lead time.

What is LIMK2 Recombinant Rabbit Monoclonal Antibody [PSH08-82] used for?

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.