SARS-CoV-2 Neutralization Antibody ELISA Kit

Specifications

Catalog number

E-EL-E608

Size / count

96 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SARS-CoV-2NeutralizationAntibody

Cross Reactivity

This kit recognizes Human SARS-CoV-2 Neutralization Antibody in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed

Target

COVID 19 Neutralization Antibody; Anti-RBD; RBD Antibody; S1RBD Antibody

Type

Competitive-ELISA

Applications

ELISA

Field of Research

SARS-CoV-2; COVID 19

Assay Principle

This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD) . After 37°C incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

15.63-500 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/joim.13523

Citation 02

https://www.mdpi.com/2073-4409/11/12/1924

Citation 03

https://www.mdpi.com/article/10.3390/healthcare11030317

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro Semi-Quantitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma.