PSIP1 Recombinant Rabbit Monoclonal Antibody [PSH02-55]

About this product

PC4 and SFRS1 interacting protein 1, also known as lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70) or transcriptional coactivator p75/p52, is a protein that in humans is encoded by the PSIP1 gene. PSIP1 has not been clearly linked to a specific cellular mechanism. The term LEDGF/p75 (Lens epithelium-derived growth factor) has entered common usage based on the initial characterization of PSIP1, however this is a misnomer, as the protein is present in most tissues and has no direct role in the development of lens epithelium. LEDGF/p75, a transcription coactivator, gained prominence as a host factor that assists HIV integration and is probably the only integrase interactor whose knock-down severely affects the HIV integration levels. The interaction between HIV integrase and human LEDGF/p75 is a promising target for anti-HIV drug discovery. LEDGF/p75 recruits MLL complexes to HOX genes to regulate their expression. LEDGF/p52 is shown to recruit splicing factors to H3K36 trimethylated chromatin to modulate alternative splicing, also regulates HOTTIP lncRNA, which is shown to regulate HOX genes in cis.

Specifications

Catalog number

HA721831

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CLL associated antigen KW 7 antibody CLL-associated antigen KW-7 antibody Dense fine speckles 70 kDa protein antibody DFS 70 antibody DFS70 antibody LEDGF antibody Lens epithelium derived growth factor antibody Lens epithelium-derived growth factor antibody MGC74712 antibody p52 antibody p75 antibody PAIP antibody PC4 and SFRS1 interacting protein 1 antibody PC4 and SFRS1 interacting protein 2 antibody PC4 and SFRS1 interacting protein antibody PC4 and SFRS1-interacting protein antibody PSIP 1 antibody PSIP 2 antibody Psip1 antibody PSIP1_HUMAN antibody PSIP2 antibody Transcriptional coactivator p75/p52 antibody

Abbreviation

PC4 and SFRS1-interacting protein CLL-associated antigen KW-7 Dense fine speckles 70 kDa protein (DFS 70) Lens epithelium-derived growth factor Transcriptional coactivator p75/p52 PSIP1 DFS70, LEDGF, PSIP2

Swiss Prot

O75475 Human, Q99JF8 Mouse, Q812D1 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human PSIP1 aa 51-530 / 530 (O75475).

Isotype

IgG

Clone

PSH02-55

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IF-Tissue

Positive Control

HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, PC-12 cell lysate, SH-SY5Y, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, rat hippocampus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes