CD105 Polyclonal Antibody

Specifications

Catalog number

D-AB-10448L-01

Size / count

25 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Human endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain. An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (l) endoglin. The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII. Similarly, they interact with Activin A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-IA/AlK-3 or BMPR-IB/AlK-6. BMP-9, however, is reported to bind endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP?7 signals in the same myoblast cell line

Abbreviation

CD105

UniProt

Q63961

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant Mouse CD105 protein expressed by E.coli

Target

AI528660; AI662476; CD 105; CD105; CD105 antigen; EGLN; END; Endoglin; Eng; FLJ41744; HHT1; ORW; ORW1; Osler Rendu Weber syndrome 1; RP11 228B15.2; S-endoglin; SN6

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IHC

Field of Research

Immunology; Stem Cells ; Cancer; Cardiovascular; Antibody Arrays; Developmental Biology; Signal Transduction

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

IHC 1:1000-1:2000

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Isotype

IgG