MDA5 Recombinant Rabbit Monoclonal Antibody [PSH03-56]

About this product

MDA5 (melanoma differentiation-associated protein 5) is a RIG-I-like receptor dsRNA helicase enzyme that is encoded by the IFIH1 gene in humans. MDA5 is part of the RIG-I-like receptor (RLR) family, which also includes RIG-I and LGP2, and functions as a pattern recognition receptor capable of detecting viruses. It is generally believed that MDA5 recognizes double stranded RNA (dsRNA) over 2000nts in length, however it has been shown that whilst MDA5 can detect and bind to cytoplasmic dsRNA, it is also activated by a high molecular weight RNA complex composed of ssRNA and dsRNA. For many viruses, effective MDA5-mediated antiviral responses are dependent on functionally active LGP2. The signaling cascades in MDA5 is initiated via CARD domain. Some observations made in cancer cells show that MDA5 also interacts with cellular RNA is able to induce an autoinflammatory response.

Specifications

Catalog number

HA722015

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CADM-140 autoantigen antibody Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody DEAD/H box polypeptide antibody Helicard antibody Helicase with 2 CARD domains antibody Hlcd antibody IDDM 19 antibody IDDM19 antibody IFIH 1 antibody Ifih1 antibody IFIH1_HUMAN antibody Interferon induced helicase C domain containing protein 1 antibody interferon induced with helicase C domain 1 antibody Interferon induced with helicase C domain protein 1 antibody Interferon-induced helicase C domain-containing protein 1 antibody Interferon-induced with helicase C domain protein 1 antibody MDA 5 antibody MDA-5 antibody Melanoma differentiation associated protein 5 antibody Melanoma differentiation-associated gene 5 antibody Melanoma differentiation-associated protein 5 antibody MGC133047 antibody Murabutide down regulated protein antibody Murabutide down-regulated protein antibody RH 116 antibody RH116 antibody RIG I like receptor 2 antibody RLR 2 antibody RNA helicase DEAD box protein 116 antibody RNA helicase-DEAD box protein 116 antibody

Abbreviation

IFIH1, MDA5, RH116

Swiss Prot

Q9BYX4 Human, Q8R5F7 Mouse,

Cellular Locus

Cytoplasm, Nucleus, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human MDA5 aa 250-1,025 / 1,025.

Isotype

IgG

Clone

PSH03-56

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

THP-1 cell lysate, HepG2 cell lysate, Jurkat cell lysate, Daudi cell lysate, Raji cell lysate, MCF7 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, A549 cell lysate, A431 cell lysate, mouse pancreas tissue lysate, rat kidney tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 117 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes