SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)

Catalog number SPD-C525d-1mg

Discontinued

Price for SPD-C525d-1mg

Size / count 1 mg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (SPD-C525d) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R, E484Q)). The mutations L452R, E484Q were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).

Specifications

Catalog number: SPD-C525d-1mg
Size / count: 1 mg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-Fc
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (Cat. No. SPD-C525d) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.8-13 ng/mL (QC tested).
Format: Powder
Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Additives: Trehalose
Molecular Weight: 51.1 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)?

The catalog number for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is SPD-C525d-1mg.

Is SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) in stock?

SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)?

SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) used for?

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.