BACE1 Recombinant Rabbit Monoclonal Antibody [PSH05-19]

About this product

Beta-secretase 1, also known as beta-site amyloid precursor protein cleaving enzyme 1, beta-site APP cleaving enzyme 1 (BACE1), membrane-associated aspartic protease 2, memapsin-2, aspartyl protease 2, and ASP2, is an enzyme that in humans is encoded by the BACE1 gene. Expression of BACE1 is observed mainly in neurons. BACE1 is an aspartic acid protease important in the formation of myelin sheaths in peripheral nerve cells: in mice the expression of BACE1 is high in the postnatal stages, when myelination occurs. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer, its cytoplasmic tail is required for the correct maturation and an efficient intracellular trafficking, but doesn't affect the activity. It is produced as a pro-enzyme, the endoproteolitc removal occurs after BACE leaves Endoplasmic reticulum, in the Golgi apparatus. In addition the pro-peptide receives additional sugars to increase the molecular mass. and the tail became a palmitoylated. The BACE1 expression is influenced by the inflammatory state: during AD the cytokines reduce the PPAR1 an inhibitor of BACE1 mRNA).

Specifications

Catalog number

HA722244

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

APP beta secretase antibody Asp 2 antibody ASP2 antibody Aspartyl protease 2 antibody BACE 1 antibody BACE antibody BACE1 antibody BACE1_HUMAN antibody Beta secretase 1 antibody Beta secretase antibody Beta site amyloid beta A4 precursor protein cleaving enzyme antibody Beta site amyloid precursor protein cleaving enzyme 1 antibody Beta site amyloid precursor protein cleaving enzyme antibody Beta site APP cleaving enzyme 1 antibody Beta site APP cleaving enzyme antibody Beta-secretase 1 antibody Beta-site amyloid precursor protein cleaving enzyme 1 antibody Beta-site APP cleaving enzyme 1 antibody FLJ90568 antibody HSPC104 antibody Memapsin 2 antibody Memapsin-2 antibody Memapsin2 antibody Membrane associated aspartic protease 2 antibody Membrane-associated aspartic protease 2 antibody Transmembrane aspartic proteinase Asp2 antibody

Abbreviation

BACE1, BACE, KIAA1149

Swiss Prot

P56817 Human, P56818 Mouse, P56819 Rat

Cellular Locus

Cell membrane, Golgi apparatus, trans-Golgi network, Endoplasmic reticulum, Endosome, Cell surface, Cytoplasmic vesicle membrane, Membrane raft, Lysosome, Late endosome, Early endosome, Recycling endosome, Cell projection, axon, dendrite.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fragment within Mouse BACE1 aa 50-350.

Isotype

IgG

Clone

PSH05-19

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Positive Control

HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IHC-Fr: 1:200 ;IF-Tissue: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 56 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes