Recombinant Human TIMP-2 Protein (hIgG1 Fc Tag)

Specifications

Catalog number

PDMH100439-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.

Abbreviation

TIMP-2

Synonyms

DDC; TIMP; metalloproteinase inhibitor; TIMP metallopeptidase inhibitor; Tissue inhibitor of metalloproteinases; CSC-21Ktissue inhibitor of metalloproteinase; TIMP2; CSC-21K; DDC8; CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; Tissue inhibitor of metalloproteinases 2; Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2 precursor; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP2; Tissue inhibitor of metalloproteinases 2; CSC 21K; CSC-21K; Tissue inhibitor of metalloproteinases 2 (TIMP-2)

UniProt

P16035

Accession Number

P16035

Expression System

Mammalian

Tag

C-hIgG1 Fc

Sequence

Cys27-Pro220

Field of Research

Cell Biology; Metabolism; Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.2 kDa

Observed Molecular Weight

50 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human