- Catalog number
- 21-159
- Size / count
- 0.1 mg
- Dry ice
- Yes
- Hazardous
- No
- Discontinued
- Yes
- Background
- <font size="3">Monkey (Cynomolgus) adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70&#730; C. The monkey (Cynomolgus) adipose tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adipose tissue is then Western analyzed by either GAPDH or &#946; -actin antibody, and the expression level is consistent with each lot.</font>
- Type
- <font size="3">Lysates</font>
- Applications
- <font size="3">Monkey Cynomolgus Adipose Lysate is suitable for :
</font><ul><li><font size="3">Western blot analysis</font></li><li><font size="3">Protein expression studies</font></li><li><font size="3">Adipose tissue research</font></li><li><font size="3">Metabolic research</font></li><li><font size="3">Comparative protein studies</font></li><li><font size="3">Non-human primate research</font></li><li><font size="3">Antibody validation</font></li><li><font size="3">Biomarker research</font></li><li><font size="3">Tissue-specific protein analysis</font></li><li><font size="3">Translational biomedical research</font></li></ul>
- Concentration
- <font size="3">2 mg/mL or better</font>
- Buffer
- <font size="3">HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.</font>
- Shipping Conditions
- <font size="3">Dry Ice</font>
- Storage Conditions
- <font size="3">Store at 2-8&#730; C for continuous use. For extended storage, freeze working aliquots at -70&#730; C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.</font>
- Applications Notes
- <font size="3">Adipose lysate is for use in Western blotting, 10 &#956; g to 20 &#956; g per lane is recommended for mini gel.</font>
- FAQ
- <font size="3"><b><font color="#0015ab">What is Monkey (Cynomolgus) Adipose Lysate?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>It is a total protein lysate prepared from Cynomolgus monkey adipose tissue for laboratory protein analysis and research applications.
<b><font color="#0015ab">What is the main application of this lysate?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>The product is tested for Western blotting (WB).
<b><font color="#0015ab">How much protein should be used for Western blotting?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>Approximately 10-20 µg per lane is recommended for a standard mini gel.
<b><font color="#0015ab">What is the protein concentration?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>The lysate has a concentration of 2 mg/mL or greater.
<br></font><div><font size="3"><b><font color="#0015ab">What species is the tissue derived from?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>The tissue source is Cynomolgus monkey.
<b><font color="#0015ab">What tissue is used?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>The lysate is prepared from adipose tissue.
<b>
<font color="#0015ab">How should Cynomolgus monkey adipose lysate be stored?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>Store at 2–8°C for continuous use. For longer-term storage, aliquot and freeze at approximately −70°C, while avoiding repeated freeze-thaw cycles.
<b><font color="#0015ab">Does the product require dry ice shipping?
</font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0015ab" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> </font></span>Yes. Dry ice shipping is required.</font></div>
- Tested Applications
- <font size="3">WB</font>
- Species
- <font size="3">Cynomolgus monkey</font>
- Physical Properties
- <font size="3">Liquid</font>