Parkin Recombinant Rabbit Monoclonal Antibody [PSH08-11]

About this product

Parkin is a 465-amino acid residue E3 ubiquitin ligase, a protein that in humans and mice is encoded by the PARK2 gene. Parkin plays a critical role in ubiquitination – the process whereby molecules are covalently labelled with ubiquitin (Ub) and directed towards degradation in proteasomes or lysosomes. Ubiquitination involves the sequential action of three enzymes. First, an E1 ubiquitin-activating enzyme binds to inactive Ub in eukaryotic cells via a thioester bond and mobilises it in an ATP-dependent process. Ub is then transferred to an E2 ubiquitin-conjugating enzyme before being conjugated to the target protein via an E3 ubiquitin ligase. There exists a multitude of E3 ligases, which differ in structure and substrate specificity to allow selective targeting of proteins to intracellular degradation.

Specifications

Catalog number

HA722952

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AR JP antibody E3 ubiquitin ligase antibody E3 ubiquitin protein ligase parkin antibody E3 ubiquitin-protein ligase parkin antibody FRA6E antibody LPRS 2 antibody LPRS2 antibody PARK 2 antibody Park2 antibody Parkin 2 antibody Parkinson disease (autosomal recessive juvenile) 2 antibody Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody Parkinson disease protein 2 antibody Parkinson juvenile disease protein 2 antibody Parkinson protein 2 E3 ubiquitin protein ligase antibody Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody PDJ antibody PRKN 2 antibody PRKN antibody PRKN2 antibody PRKN2_HUMAN antibody Ubiquitin E3 ligase PRKN antibody

Abbreviation

PRKN, PARK2

Swiss Prot

O60260 Human, Q9WVS6 Mouse, Q9JK66 Rat

Cellular Locus

Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within human Parkin aa 1-200.

Isotype

IgG

Clone

PSH08-11

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue, IF-Cell, FC, IP, IHC-Fr

Positive Control

SH-SY5Y cell lysate, 293T cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue, SH-SY5Y, Neuro-2a, C6.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;IF-Cell: 1:50-1:100 ;FC: 1:1,000 ;IP: IP: 1-2μg/sample ;IHC-Fr: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 52 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes