PDK4 Polyclonal Antibody

Specifications

Catalog number

AN006800L-01

Size / count

25 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Abbreviation

PDK4

UniProt

Q16654

Host

Rabbit

Reactivity

Mouse; Rat

Immunogen

Recombinant Human PDK4 protein expressed by E.coli

Target

PDK; PDHK; Pyruvate dehydrogenase lipoamide kinase isozyme; PDK4PDHK; PDK4; FLJ40832; mitochondrial; Pyruvate dehydrogenase lipoamide kinase isozyme 4; PDK4PDHK4; PDHK4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial; Pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoenzyme 4; Pyruvate dehydrogenase kinase isoform 4; Pyruvate dehydrogenase kinase isozyme 4; Pyruvate dehydrogenase kinase isozyme 4 mitochondrial

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB

Field of Research

Signal Transduction; Metabolism

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000

Buffer

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

46 kDa

Observed Molecular Weight

50-55 kDa

Isotype

IgG