Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27]

Catalog number HA721443

Price for HA721443

Size / count 100 µL

About this product

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial is an enzyme that in humans is encoded by the PDHA1 gene.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.

Specifications

Catalog number: HA721443
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: ODPA_HUMAN antibody PDH antibody PDHA antibody PDHA1 antibody PDHCE1A antibody PDHE1 A type I antibody PDHE1-A type I antibody PHE1A antibody Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody Pyruvate Dehydrogenase E1 alpha antibody Pyruvate dehydrogenase E1 component subunit alpha antibody Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody Pyruvate Dehydrogenase E1-alpha subunit antibody
Abbreviation: PDHA1, PHE1A
Swiss Prot: P08559 Human, P35486 Mouse, P26284 Rat
Cellular Locus: Mitochondrion matrix.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human PDHA1 protein.
Isotype: IgG
Clone: JE58-27
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, ICC, IHC-P
Positive Control: A549 cell lysate, Jurkat cell lysate, HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, human kidney tissue lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat kidney tissue lysate, mouse kidney tissue lysate, Jurkat, human renal clear cell carcinoma tissue, mouse brain tissue, rat colon tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;ICC: 1:100 ;IHC-P: 1:500-1:2,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 43 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27]?

The catalog number for Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27] is HA721443.

What sizes are available for Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27]?

Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27] is available in the following size(s): 100 µL.

Is Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27] in stock?

Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-PDHA1 (S293) Recombinant Rabbit Monoclonal Antibody [JE58-27] used for?

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.