SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)

Catalog number SPD-C525d-100ug

Discontinued

Price for SPD-C525d-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (SPD-C525d) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R, E484Q)). The mutations L452R, E484Q were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).

Specifications

Catalog number: SPD-C525d-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-Fc
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (Cat. No. SPD-C525d) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.8-13 ng/mL (QC tested).
Format: Powder
Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Additives: Trehalose
Molecular Weight: 51.1 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)?

The catalog number for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is SPD-C525d-100ug.

Is SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) in stock?

SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)?

SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike RBD Protein (L452R, E484Q), Fc Tag (MALS verified) used for?

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.