TRF2 Recombinant Rabbit Monoclonal Antibody [JE37-57]

About this product

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Specifications

Catalog number

HA721297

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Telomeric DNA binding protein antibody Telomeric DNA-binding protein antibody Telomeric repeat binding factor 2 antibody Telomeric repeat binding protein 2 antibody Telomeric repeat-binding factor 2 antibody TERF 2 antibody Terf2 antibody TERF2_HUMAN antibody TRBF 2 antibody TRBF2 antibody TRF 2 antibody TRF2 antibody TTAGGG repeat binding factor 2 antibody TTAGGG repeat-binding factor 2 antibody

Abbreviation

TERF2, TRBF2, TRF2

Swiss Prot

Q15554 Human

Cellular Locus

Nucleus, Chromosome, telomere.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human TRF2 aa 1-100 / 542.

Isotype

IgG

Clone

JE37-57

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

SiHa cell lysate, Jurkat cell lysate, human colon carcinoma tissue, human testis tissue, SiHa.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 20ug/mL

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes