Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) Recombinant Rabbit Monoclonal Antibody [PSH05-73]

About this product

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation . ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers. Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function. Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation. Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation.

Specifications

Catalog number

HA722440

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Villin 2 ezrin antibody CVIL antibody CVL antibody Cytovillin 2 antibody Cytovillin antibody DKFZp762H157 antibody Epididymis secretory protein Li 105 antibody EZR antibody EZRI_HUMAN antibody Ezrin antibody FLJ26216 antibody HEL S 105 antibody MGC1584 antibody p81 antibody VIL 2 antibody VIL2 antibody Villin 2 (ezrin) antibody Villin 2 antibody Villin-2 antibody Villin2 antibody CB567 antibody CG12537 antibody DFNB24 antibody ESP10 antibody Hh-induced MATH and BTB domain-containing protein antibody HIB antibody Moesin-B antibody Protein roadkill antibody RADI_HUMAN antibody Radixin antibody RDX antibody Epididymis luminal protein 70 antibody HEL70 antibody Membrane organizing extension spike protein antibody Membrane-organizing extension spike protein antibody MOES_HUMAN antibody Moesin antibody Moesin/anaplastic lymphoma kinase fusion protein antibody Msn antibody MSN/ALK fusion antibody

Abbreviation

Ezrin Cytovillin Villin-2 p81 EZR VIL2 Radixin RDX Moesin Membrane-organizing extension spike protein MSN

Swiss Prot

P15311 Human, P26038 Human, P35241 Human, P26040 Mouse, P26041 Mouse, P26043 Mouse, P31977 Rat, O35763 Rat

Cellular Locus

Cell membrane. Cell projection. Cytoplasm. Cytoskeleton. Membrane

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein.

Isotype

IgG

Clone

PSH05-73

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate, mouse liver tissue, rat liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 69 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes