Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66]

Catalog number HA722848

Price for HA722848

Size / count 100 µL

About this product

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

Specifications

Catalog number: HA722848
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: H3 histone family member E pseudogene antibody H3 histone family, member A antibody H3/A antibody H31_HUMAN antibody H3F3 antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody histone 1, H3a antibody Histone cluster 1, H3a antibody Histone H3 3 pseudogene antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody H3K27me3
Abbreviation: Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l
Swiss Prot: P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P68433 Mouse, P84228 Mouse, Q6LED0 Rat
Cellular Locus: Nucleus, Chromosome.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Monkey
Immunogen: Synthetic peptide corresponding to residues surrounding mono-methyl Lys79 of human histone H3 protein.
Isotype: IgG
Clone: PSH07-66
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC, ChIP
Positive Control: NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa cell lysate, HeLa, C2C12, C6, mouse brain tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 15 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66]?

The catalog number for Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66] is HA722848.

What sizes are available for Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66]?

Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66] is available in the following size(s): 100 µL.

Is Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66] in stock?

Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Histone H3 (mono methyl K79) Recombinant Rabbit Monoclonal Antibody [PSH07-66] used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.