Recombinant Human ANG2 Protein (His Tag)

Specifications

Catalog number

PDMH100179-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

Abbreviation

ANG2

Synonyms

Angiopoitin; ANGP; Ang; ANGPT2; AGPT2; ANG2; AGPT 2; ANG 2; angiopoietin 2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGP2; ANGPT 2; Tie2 ligand; Tie2-ligand; Angiopoietin-2; ANG-2; Agpt2; ANG2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin2; ANGPT 2; Angpt2; Tie2 ligand

UniProt

O15123

Accession Number

O15123

Expression System

Mammalian

Tag

C-His

Sequence

Asp68-Phe496

Field of Research

Cancer; Cardiovascular; Developmental Biology; Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.1 kDa

Observed Molecular Weight

100-120 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human