Phospho-p70 S6 Kinase (T389) Recombinant Rabbit Monoclonal Antibody [PSH02-23]

About this product

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Specifications

Catalog number

HA721803

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

70 kDa ribosomal protein S6 kinase 1 antibody p70 alpha antibody p70 S6 kinase alpha antibody p70 S6K antibody p70 S6KA antibody p70 (S6K) alpha antibody p70s6kA antibody PS6K antibody Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody ribosomal protein S6 kinase beta 1 antibody ribosomal protein S6 kinase I antibody Ribosomal protein S6 kinase, 70-kD antibody RPS6KB1 antibody S6K antibody S6K1 antibody Serine/threonine kinase 14 alpha antibody Serine/threonine protein kinase 14A antibody STK14A antibody

Abbreviation

RPS6KB1, STK14A

Swiss Prot

P23443 Human, Q8BSK8 Mouse,

Cellular Locus

Synapse, synaptosome, Mitochondrion outer membrane, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human P70 S6 Kinase aa 371-420 / 525.

Isotype

IgG

Clone

PSH02-23

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

MCF7 cell lysate, MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate, human breast carcinoma tissue, MCF7 starved for 4 hours then treated with 200ng/mL EGF for 15 minutes cell lysate, C6 cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, human liver carcinoma tissue, 293T cell lysate, 293T serum starved overnight cell lysate, 293T serum starved overnight then treated with 100nM Insulin for 15 minutes cell lysate, MCF7 cells treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 59 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes