3mC Recombinant Rabbit Monoclonal Antibody [PSH03-21]

About this product

Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.

Specifications

Catalog number

HA721957

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Host

Rabbit

Species Reactivity

Universal

Immunogen

3-mc-OVA

Isotype

IgG

Clone

PSH03-21

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IF-Cell, Dot Blot

Positive Control

U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour.

Concentration

1 mg/mL

Dilution

IF-Cell: 1:100 ;Dot Blot: 1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes