Phospho-EEF2 (T56) Recombinant Rabbit Monoclonal Antibody [PSH06-36]

About this product

Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. It is the archaeal and eukaryotic counterpart of bacterial EF-G. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the ribosome. This protein is completely inactivated by EF-2 kinase phosphorylation. aEF2/eEF2 found in most archaea and eukaryotes, including humans, contains a post translationally modified histidine diphthamide. It is the target of diphtheria toxin (from Corynebacterium diphtheriae), and exotoxin A (from Pseudomonas aeruginosa). The inactivation of EF-2 by toxins inhibits protein production in the host, causing symptoms due to loss of function in affected cells.

Specifications

Catalog number

HA722670

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

EEF 2 antibody Eef2 antibody EF-2 antibody EF2 antibody EF2_HUMAN antibody Elongation factor 2 antibody Eukaryotic translation elongation factor 2 antibody Polypeptidyl tRNA translocase antibody SCA26 antibody

Abbreviation

EEF2, EF2

Swiss Prot

P13639 Human, P58252 Mouse, P05197 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr56 of human EEF2.

Isotype

IgG

Clone

PSH06-36

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

HeLa starved for 24 hours then add 10μM forskolin for 1 hour cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved for 24 hours then add 10μM forskolin for 1 hour cell lysate, PC-12 treated with 20μM forskolin for 1 hour then add 100nM Calyculin A for 30 minutes cell lysate, HepG2 cell lysate, rat liver tissue, HeLa cells starved for 24 hours then add 10μM forskolin for 1 hour, PC-12 cells treated with 20μM forskolin for 1 hour then add 100nM Calyculin A for 30 minutes.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:500 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 95 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes