Phenylalanine (Phe) Colorimetric Assay Kit

Specifications

Catalog number

E-BC-K846-M

Size / count

96 T (40 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Colorimetric method

Field of Research

Liver and Renal Function; Amino Acids and Proteins

Assay Principle

Phenylalanine (Phe), chemically known as 2-amino-3-phenylpropionic acid, is an essential amino acid involved in a variety of biosynthetic processes. In the body, most Phe is oxidized to tyrosine by phenylalanine hydroxylase, and together with tyrosine, it synthefies important neurotransmitters and hormones, and participates in glucose metabolism and fat metabolism. Phenylalanine is catalyzed by enzyme , and the chromogenic agent has an absorption peak at 450 nm. The Phe content of the sample can be calculated by measuring the OD value of the chromogenic substance at 450 nm.

Assay Performance Time

50 min

Sample Type

Serum; Plasma; Urine; Animal and plant tissue; Cell; Cell culture supernatant

Detection Range

4.25-500.00 μmol/L

Sensitivity

4.25 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

2.4-4.0%

Intra CV

0.9-1.6%

Detection Instrument

Microplate reader