KPNB1 Recombinant Rabbit Monoclonal Antibody [PSH01-60]

About this product

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In association with IPO7, mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.

Specifications

Catalog number

HA721703

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

IMB 1 antibody IMB1 antibody IMB1_HUMAN antibody Impnb antibody Importin 90 antibody Importin beta 1 antibody Importin beta 1 subunit antibody Importin subunit beta-1 antibody Importin-90 antibody IPOB antibody Karyopherin beta 1 antibody Karyopherin beta 1 subunit antibody Karyopherin subunit beta-1 antibody KPNB 1 antibody Kpnb1 antibody MGC2155 antibody MGC2156 antibody MGC2157 antibody NTF 97 antibody NTF97 antibody NTF97/Importin beta antibody Nuclear factor P97 antibody Pore targeting complex 97 kDa subunit antibody PTAC97 antibody

Abbreviation

Importin subunit beta-1 Importin-90 Karyopherin subunit beta-1 Nuclear factor p97 Pore targeting complex 97 kDa subunit (PTAC97) KPNB1 NTF97

Swiss Prot

Q14974 Human, P70168 Mouse, P52296 Rat

Cellular Locus

Cytoplasm. Nucleus envelope.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human KPNB1 aa 1-550.

Isotype

IgG

Clone

PSH01-60

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue, IP

Positive Control

HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, Jurkat cell lysate, K-562 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse spleen tissue lysate, mouse testis tissue lysate, rat spleen tissue lysate, rat testis tissue lysate, human colon tissue, human kidney tissue, human testis tissue, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 97.2 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes