Histone H3 (acetyl K9) Recombinant Rabbit Monoclonal Antibody [PSH04-47] - ChIP Grade

About this product

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

Specifications

Catalog number

HA722132

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

H3 histone family, member A antibody H3/A antibody H31_HUMAN antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody histone 1, H3a antibody Histone cluster 1, H3a antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody H3K9AC antibody

Abbreviation

Hist1h3a, Hist1h3b,H3K4Ac

Swiss Prot

P68431 human, P84243 human, Q16695 human, Q6NXT2 human, Q71DI3 human, P68433 mouse, P84228 mouse, Q6LED0 rat

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Histone H3 (acetyl K9) aa 1-50.

Isotype

IgG

Clone

PSH04-47

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IF-Tissue, FC, ChIP, Dot Blot, IP

Positive Control

HeLa cell lysate, HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, C6, HeLa cells treated with 500ng/mL TSA for 4 hours, human stomach tissue, mouse colon tissue, rat colon tissue, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:5,000-1:15,000 ;IHC-P: 1:2,000 ;IF-Tissue: 1:200 ;FC: 1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;Dot Blot: 1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes