Purified Anti-Human CD74 Antibody[LL1]

Specifications

Catalog number

E-AB-F14210P-01

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production.

Abbreviation

CD74

Gene ID

972

UniProt

P04233

Host

Mouse

Reactivity

Human

Immunogen

Recombinant Human CD74 protein

Target

HLA class II histocompatibility antigen gamma chain; HLADG; Ia antigen-associated invariant chain; Ia-GAMMA II; Ii; p33; CD74 HLA-DR antigens-associated invariant chain; DHLAG; CD74; invariant chain; CD 74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex; CD74 molecule; class II antigen-associated) ; class II invariant chain; CLIP; Gamma chain of class II antigens; HG2A; HLA DR antigens associated invariant chain; HLA DR gamma; HLADR antigens associated invariant chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen associated invariant chain; Ia associated invariant chain; Ia gamma; Ia-GAMMA; Invariant polypeptide of major histocompatibility complex class II antigen associated; la-gamma; major histocompatibility complex; Major histocompatibility complex class II invariant chain; MHC HLA DR gamma chain; MHC HLA-DR gamma chain; p35; Protein 41; CD74; DHLAG; HLA class II histocompatibility antigen gamma chain; HLADG; Ia antigen-associated invariant chain; p33; CD 14; CD_antigen=CD14; CD14; CD14 antigen; CD14 molecule; LPS-R; Mo2; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14 urinary form; membrane-bound form; Myeloid cell specific leucine rich glycoprotein; Myeloid cell-specific leucine-rich glycoprotein

Clonality

Monoclonal

Clone

LL1

Conjugation

Unconjugated

Applications

FCM; WB

Field of Research

Immunology

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Calculated Molecular Weight

34 kDa

Observed Molecular Weight

30-35 kDa

Isotype

Mouse IgG1, κ