SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag

Catalog number SPD-C52Hf-100ug

Discontinued

Price for SPD-C52Hf-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 S protein RBD (F490L), His Tag (SPD-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (F490L)).

Specifications

Catalog number: SPD-C52Hf-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 26.6 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag?

The catalog number for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is SPD-C52Hf-100ug.

Is SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag in stock?

SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag?

SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag?

Estimated delivery time for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag used for?

SARS-CoV-2 S protein RBD (F490L), His Tag (SPD-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (F490L)).

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.