AF/LE Purified Anti-Mouse CD3e Antibody[500A2]

Specifications

Catalog number

AN003420-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098) . In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region

Abbreviation

CD3e

Gene ID

12501

UniProt

P22646

Host

Syrian Hamster

Reactivity

Mouse

Immunogen

Recombinant Mouse CD3e protein

Target

T3E; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; Cd3e; T3; CD3epsilon

Clonality

Monoclonal

Clone

500A2

Conjugation

None (AF/LE)

Applications

FCM

Purification

>98%, Protein A/G purified

Concentration

≥ 1 mg/mL

Dilution

66

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Isotype

Syrian Hamster IgG