Cell Mitochondrial Complex IV (Cytochrome C Oxidase) Activity Assay Kit

Specifications

Catalog number

E-BC-K837-M-01

Size / count

48 T (46 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

Cytochrome C Oxidase

Type

Colorimetric method

Field of Research

Mitochondria Function

Assay Principle

Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.

Assay Performance Time

35 min

Sample Type

Cell

Detection Range

0.52-30.9 U/L

Sensitivity

0.52 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

4.0-9.5%

Intra CV

3.0-5.5%

Detection Instrument

Microplate reader