CD2 / Lymphocyte Function Antigen 2 (LFA-2) (UMCD2), CF594 conjugate, 0.1mg/mL

About this product

CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC940201-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Erythrocyte receptor; LFA-2; LFA-3 receptor; Ly-37; Lymphocyte function antigen 2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD2

Gene ID

914

NCBI Gene ID

523500

UniProt

P06729

Swiss Prot

P06729

Host

Mouse

Species Reactivity

Human

Reactivity

CD2|LFA-2|Lymphocyte Function Antigen 2

Immunogen

Human Thymocytes/Sezary T cells

Target Antigen

CD2 | LFA-2 | Lymphocyte Function Antigen 2

Clonality

Monoclonal

Clone

UMCD2

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |IF (published) |IHC, FFPE (published)

Positive Control

293T or Jurkat cells. Lymph node and tonsil

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~50 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Transplant Proc (1987) 19(1):277-8. (functional studies, IP) Fert Stert (2008) 90,(3), P849.E5-849.E8. (IHC, frozen) eLife. 2018; 7: e31657. (IF, FFPE) Am J Transplant. 2019;20:88-100. (Flow)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF594