Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL

Catalog number BNUB0252-100

Price for BNUB0252-100

Size / count 1x 100 µL

About this product

This MAb recognizes the 56. 5 kDa (CK10) ; 50 kDa (CK14) ; 50 kDa (CK15) ; 48 kDa (CK16) ; 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e. g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB0252-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: K1B; KRT1B; Keratin; type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT77
Gene ID: 374454
NCBI Gene ID: 334989
UniProt: Q7Z794
Swiss Prot: Q7Z794
Host: Mouse
Species Reactivity: Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat, Turtle
Reactivity: Cytokeratin, Acidic
Immunogen: Human epidermal keratin
Target Antigen: Cytokeratin, Acidic
Clonality: Monoclonal
Clone: AE-1
Type: Primary
Source: Animal
Origin: Animal
Field of Research: Cancer, Cytoskeleton
Positive Control: Skin or squamous cell carcinoma (IHC).
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 40-56.5 kDa
References & Citations: Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. |Tseng SCG et. al. Cell 1982; 30361
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL?

The catalog number for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL is BNUB0252-100.

What sizes are available for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL?

Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL in stock?

Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL?

Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL?

Estimated delivery time for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL?

The safety data sheet for Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (AE-1), 0.2mg/mL used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.