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Product details

Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0265-100

Price for BNCB0265-100

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Size / count 1x 100 µL

About this product

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNCB0265-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
P-Tyr
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
UniProt
Not Applicable
Swiss Prot
Not Applicable
Host
Mouse
Species Reactivity
Pan species
Reactivity
Phosphotyrosine
Immunogen
Phosphotyrosine conjugated to BSA
Target Antigen
Phosphotyrosine
Clonality
Monoclonal
Clone
PY265
Type
Primary
Source
Animal
Origin
Animal
Positive Control
MCF-7, MDA-231, T47-D cells. Breast carcinoma.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
Depends upon the phosphorylated target
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
Biotin

Frequently Asked Questions

What is the catalog number for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL?

The catalog number for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL is BNCB0265-100.

What sizes are available for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL?

Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL in stock?

Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL?

Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029c061270361e10226b/msds/bncb0265-100.pdf.

What is Phosphotyrosine (P-Tyr) (PY265), Biotin conjugate, 0.1mg/mL used for?

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

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