CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL — image 1 of 2

Product details

CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL

Catalog number BNC940268-500

Price for BNC940268-500

Size / count 1x 500 µL

About this product

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes, and is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and CD3 is predominantly intracytoplasmic in cortical thymus. Anti-CD3 clone OKT3 has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. In the crystal structure of the antibody-receptor complex, clone OKT3 was found to bind a conformational epitope on CD3e dominated by a linear stretch in the F-G loop of the subunit. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC940268-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CD3 epsilon; CD3 TCR complex; CD3e antigen epsilon polypeptide (TiT3 complex) ; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD3E
Gene ID: 916
NCBI Gene ID: 3003
UniProt: P07766
Swiss Prot: P07766
Host: Mouse
Species Reactivity: Human
Reactivity: CD3e
Immunogen: Human PBMCs
Target Antigen: CD3e
Clonality: Monoclonal
Clone: OKT3
Type: Primary
Source: Animal
Origin: Animal
Applications: Functional studies (published) |IHC, frozen (published) |Flow, surface (verified) |IF (verified) |IP (published)
Positive Control: Jurkat cells. Tonsil and lymph node
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 23 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1992) 267(17): 11685-11688. (immunoprecipitation) PNAS USA (2004) 101(20): 7675-7680. (crystal structure of antibody/antigen complex) J Immunol (2005) 174:3773-3780. (IHC, frozen) Blood (2011) 117: 5463-5472. (in vivo cell depletion) J Immunol (2012) 188:5538-5546. (functional studies)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL?

The catalog number for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL is BNC940268-500.

What sizes are available for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL?

CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL in stock?

CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL?

CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL?

The safety data sheet for CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD3e (T-Cell Marker) (OKT3), CF594 conjugate, 0.1mg/mL used for?

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.